1st screening
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Information retrieval of samples
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NMR titration experiments by using proteins which regulate tight junctions
(This experiment can analyze mixtures solubilized by water or DMSO)
2nd screening
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By using Caco-2 cells exposed to compounds
Barrier function evaluation (Transepithelial electric resistance; TER)
(Additional analysis) permeability evaluation by using model substrates
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(Additional service) by using MDCK II cells exposed to compounds
Immunocytochemically characterization of subcellular localization of tight junction proteins