1st screening

  • Information retrieval of samples

  • NMR titration experiments by using proteins which regulate tight junctions

(This experiment can analyze mixtures solubilized by water or DMSO)


2nd screening

  • By using Caco-2 cells exposed to compounds

Barrier function evaluation (Transepithelial electric resistance; TER)

(Additional analysis) permeability evaluation by using model substrates

  • (Additional service) by using MDCK II cells exposed to compounds

Immunocytochemically characterization of subcellular localization of tight junction proteins


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